Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: PIL5
Entrez Gene ID: 816538
Gene Name: phytochrome interacting factor 3-like 5
Gene Aliases: AT2G20180, PHY-INTERACTING FACTOR 1, PIF1, T2G17.2, T2G17_2, phytochrome interacting factor 3-like 5
Chromosome Location: Chr.2: 8704024 - 8707009 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001202630.2	NP_001189559.1	5 - 6	1180	135
	NM_001335663.1	NP_001318252.1	5 - 6	1175	135
	NM_001335664.1	NP_001323902.1	4 - 5	1307	135
	NM_001335665.1	NP_001323903.1	4 - 5	1270	135
	NM_001335666.1	NP_001323904.1	4 - 5	1411	135
	NM_001335667.1	NP_001323905.1	4 - 5	1374	135
	NM_179665.3	NP_849996.1	5 - 6	1138	135
GenBank mRNA	AF488560.2	-	4 - 5	945	135
GenBank mRNA	BT029775.1	-	3 - 4	732	135