Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: THI1
Entrez Gene ID: 835567
Gene Name: thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
Gene Aliases: AT5G54770, MBG8.3, MBG8_3, THI1 PROTEIN, THI4, THIAMINE4, THIAZOLE REQUIRING, TZ
Chromosome Location: Chr.5: 22246519 - 22248332 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_124858.4	NP_200288.1	1 - 2	516	56
GenBank mRNA	AF419604.1	-	1 - 2	440	56
	AF428355.1	-	1 - 2	440	56
	AF428385.1	-	1 - 2	440	56
	AY054216.1	-	1 - 2	440	56
	AY058094.1	-	1 - 2	440	56
	AY143082.1	-	1 - 2	401	56
	U17589.1	-	1 - 2	422	56