The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001344740.1 | NP_001318754.1 | 2 - 2 | 197 | 119 |
NM_124141.4 | NP_199578.2 | 1 - 1 | 326 | 119 | |
GenBank mRNA | AY138461.1 | - | 1 - 1 | 43 | 119 |