Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: SkpA
Entrez Gene ID: 31016
Gene Name: SKP1-related A
Gene Aliases: CG16983, Dmel\CG16983, Dmel_CG16983, EG:115C2.4, SKP1, SKPA, Skp, Skp1, anon-WO03040301.234, anon-WO03040301.236, anon-WO03040301.238, dSKP1, dSkip-1, dSkp-1, dSkpA, dskpA, l(1)G0037, l(1)G0058, l(1)G0109, l(1)G0389, skpA, spkA
Chromosome Location: Chr.X: 656114 - 657899 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0025637

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001038729.3	NP_001033818.1	1 - 1	1371	127
	NM_001297826.1	NP_001284755.1	1 - 1	653	127
	NM_058042.5	NP_477390.1	2 - 2	914	127
	NM_166856.3	NP_726690.1	2 - 2	762	127
	NM_166857.3	NP_726691.1	2 - 2	877	127
	NM_166858.3	NP_726692.1	2 - 2	1299	127
	NM_166859.3	NP_726693.1	2 - 2	696	127
	NM_166860.3	NP_726694.1	2 - 2	740	127
	NM_166861.3	NP_726695.1	2 - 2	672	127
GenBank mRNA	AY069297.1	-	1 - 1	1333	127
	BT099582.1	-	2 - 2	700	127
	BT099610.1	-	2 - 2	857	127