Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: RpII15
Entrez Gene ID: 41741
Gene Name: RNA polymerase II 15kD subunit
Gene Aliases: CG3284, Dmel\CG3284, Dmel_CG3284, H5, Pol II, PolII, RNA Pol II, RNA pol II, RNA polII, RNAP, RNAP II, RNApolII, RPII15, RpII215, Rpb9, dRPB9, l(3)88Be, l(3)Z23, pol II, polII
Chromosome Location: Chr.3R: 14309089 - 14310178 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0004855

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001260170.2	NP_001247099.1	2 - 2	538	133
	NM_001300397.1	NP_001287326.1	2 - 2	545	133
	NM_169575.3	NP_731898.1	2 - 2	545	133