The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_033271.2 | NP_150374.2 | 2 - 2 | 280 | 269 |
| XM_005268188.3 | XP_005268245.1 | 1 - 1 | 448 | 269 | |
| GenBank mRNA | BC042525.1 | - | 2 - 2 | 250 | 147 |
| DB456488.1 | - | 2 - 2 | 284 | 269 | |