The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001167856.1 | NP_001161328.1 | 31 - 31 | 10466 | 145 |
| NM_018183.3 | NP_060653.3 | 31 - 31 | 10463 | 145 | |
| XM_005253572.3 | XP_005253629.1 | 32 - 32 | 10604 | 145 | |
| XM_011538534.2 | XP_011536836.1 | 31 - 31 | 10502 | 145 | |
| XM_011538535.2 | XP_011536837.1 | 31 - 31 | 10504 | 145 | |
| XM_017019556.1 | XP_016875045.1 | 32 - 32 | 10588 | 145 | |
| XM_017019557.1 | XP_016875046.1 | 32 - 32 | 10595 | 145 | |
| XM_017019558.1 | XP_016875047.1 | 33 - 33 | 10658 | 145 | |
| XM_017019559.1 | XP_016875048.1 | 32 - 32 | 10725 | 145 | |