The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_002139.3 | NP_002130.2 | 5 - 5 | 724 | 101 |
| NR_028476.1 | 4 - 4 | 552 | 101 | ||
| NR_028477.1 | 5 - 5 | 759 | 101 | ||
| GenBank mRNA | AK091520.1 | - | 4 - 4 | 498 | 101 |
| AK096015.1 | - | 2 - 2 | 630 | 101 | |
| AK304573.1 | - | 5 - 5 | 704 | 101 | |
| AK309911.1 | - | 5 - 5 | 679 | 101 | |
| AY464692.1 | - | 4 - 4 | 514 | 101 | |
| BC006550.2 | - | 5 - 5 | 660 | 101 | |
| BC007435.2 | - | 5 - 5 | 660 | 101 | |
| BX647131.1 | - | 4 - 4 | 495 | 101 | |
| DA925805.1 | - | 4 - 4 | 497 | 101 | |
| Z23064.1 | - | 4 - 4 | 525 | 101 | |