The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_002520.6 | NP_002511.1 | 11 - 11 | 1110 | 143 |
| NM_199185.3 | NP_954654.1 | 10 - 10 | 1023 | 143 | |
| XM_011534564.1 | XP_011532866.1 | 10 - 10 | 930 | 143 | |
| GenBank mRNA | AK290652.1 | - | 11 - 11 | 973 | 143 |
| AY347529.1 | - | 12 - 12 | 1000 | 143 | |
| AY740640.1 | - | 11 - 11 | 934 | 143 | |
| BC002398.2 | - | 11 - 11 | 952 | 143 | |
| BC003670.1 | - | 1 - 1 | 667 | 143 | |
| BC008495.1 | - | 11 - 11 | 962 | 143 | |
| BC009623.2 | - | 10 - 10 | 904 | 143 | |
| BC014349.1 | - | 11 - 11 | 961 | 143 | |
| BC016716.1 | - | 11 - 11 | 964 | 143 | |
| BC016768.1 | - | 11 - 11 | 964 | 143 | |
| BC016824.1 | - | 11 - 11 | 964 | 143 | |
| BC020467.1 | - | 11 - 11 | 951 | 143 | |
| BC021668.1 | - | 11 - 11 | 965 | 143 | |
| BC021983.1 | - | 11 - 11 | 963 | 143 | |
| BC050628.1 | - | 11 - 11 | 951 | 143 | |
| BU661768.1 | - | 7 - 7 | 599 | 143 | |
| J02590.1 | - | 2 - 2 | 229 | 143 | |
| M23613.1 | - | 11 - 11 | 958 | 143 | |
| M26697.1 | - | 11 - 11 | 955 | 143 | |
| M28699.1 | - | 11 - 11 | 952 | 143 | |
| M31004.1 | - | 4 - 4 | 229 | 143 | |