Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
H2AFY
Entrez Gene ID
9555
Gene Name
H2A histone family member Y
Gene Aliases
H2A.y, H2A/y, H2AF12M, MACROH2A1.1, mH2A1, macroH2A1.2
Chromosome Location
Chr.5: 135334381 - 135399887 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq XM_005272132.1 XP_005272189.1 1 - 1 106
XM_005272134.3 XP_005272191.1 1 - 1 106
XM_005272135.3 XP_005272192.1 1 - 1 106
XM_011543731.1 XP_011542033.1 1 - 1 106
XM_011543732.1 XP_011542034.1 1 - 1 106
XM_011543733.2 XP_011542035.1 1 - 1 106
XM_011543734.2 XP_011542036.1 1 - 1 106
XM_011543735.2 XP_011542037.1 1 - 1 106
XM_017010067.1 XP_016865556.1 1 - 1 106
XM_017010068.1 XP_016865557.1 1 - 1 106
XM_017010069.1 XP_016865558.1 1 - 1 106


Non Gene Transcripts

Interrogated Sequence Translated Protein Exon Boundary Assay Location IMAGE Clone ID Transcript Location Amplicon Length
GenBankmRNA CX167042.1 - 1 Chr. 5 - 135389107 - 135399533 [-] on Build GRCh38 106
GenBankmRNA DA148558.1 - 1 Chr. 5 - 135399165 - 135399622 [-] on Build GRCh38 106
GenBankmRNA DA274581.1 - 1 157 Chr. 5 - 135399286 - 135399842 [+] on Build GRCh38 106
GenBankmRNA DA920818.1 - 1 148 Chr. 5 - 135399280 - 135399836 [+] on Build GRCh38 106
GenBankmRNA BI560542.1 - 1 82 Chr. 5 - 135399347 - 135400052 [+] on Build GRCh38 106
GenBankmRNA BQ948280.1 - 134 Chr. - - [-] on Build 106
GenBankmRNA AK092789.1 - 1 148 Chr. 5 - 135399280 - 135401292 [+] on Build GRCh38 106


Adjacent Gene Details

Gene Name Gene Symbol Alias Distance Relative Direction Location Chromosome
Upstream chromosome 5 open reading frame 66 C5orf66 -54.6kb Sense Chr. - 135033280 - 135344680 on Build GRCh38
Downstream dendritic cell associated nuclear protein DCANP1 C5orf20, DCNP1 42.9kb Anti-sense Chr. - 135444214 - 135447348 on Build GRCh38

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