The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_013990866.2 | XP_013846320.2 | - | 2151 | 92 |
| XM_021080354.1 | XP_020936013.1 | - | 2085 | 92 | |
| XM_021080355.1 | XP_020936014.1 | - | 2168 | 92 | |
| XM_021080356.1 | XP_020936015.1 | - | 2123 | 92 | |
| XM_021080357.1 | XP_020936016.1 | - | 1948 | 92 | |
| GenBank mRNA | AF132070.1 | - | 2 - 2 | 249 | 92 |
| AK234808.1 | - | 6 - 6 | 807 | 92 | |
| AK391881.1 | - | 16 - 16 | 2134 | 92 | |