The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001105478.1 | NP_001098948.1 | 1 - 1 | 45 | 141 |
GenBank mRNA | BC149580.1 | - | 1 - 1 | 45 | 141 |
EU846101.1 | - | 1 - 1 | 45 | 141 |