The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001002993.2 | NP_001002993.2 | - | 586 | 156 |
| XM_022422515.1 | XP_022278223.1 | - | 586 | 156 | |
| GenBank mRNA | U91844.2 | - | - | 586 | 156 |