The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001003291.1 | NP_001003291.1 | 5 - 5 | 1166 | 124 |
| GenBank mRNA | L31625.1 | - | 5 - 5 | 1145 | 124 |
| M68524.1 | - | 3 - 3 | 596 | 124 | |