The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001171773.2 | NP_001165244.2 | 3 - 3 | 440 | 130 |
| GenBank mRNA | GQ303574.1 | - | 3 - 3 | 440 | 130 |
| GQ303577.1 | - | 2 - 2 | 617 | 130 | |