The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | XM_022421425.1 | XP_022277133.1 | 4 - 4 | 344 | 244 |
XM_850110.5 | XP_855203.1 | 2 - 2 | 229 | 149 |