The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005627586.3 | XP_005627643.1 | 3 - 3 | 192 | 188 |
| XM_005627587.3 | XP_005627644.1 | 4 - 4 | 353 | 188 | |
| XM_022426311.1 | XP_022282019.1 | 3 - 3 | 216 | 188 | |
| XM_849202.5 | XP_854295.2 | 3 - 3 | 513 | 188 | |