Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pka-R1
Entrez Gene ID: 40305
Gene Name: Protein kinase, cAMP-dependent, regulatory subunit type 1
Gene Aliases: 18304, CG12452, CG18677, CG3263, CG42341, CdkR, DRI, Dmel\CG42341, Dmel_CG42341, PKA, PKA R1, PKA-R1, PKA-RI, PKAR, PKARI, PKAi, PKAr, PKa-R1, Pka-RI, PkaR, RI, dPKA-RI, dr[[1]], pKA, pkA, pka-R1
Chromosome Location: Chr.3L: 20816118 - 20836048 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0259243

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001014593.3	NP_001014593.1	2 - 3	1072	67
	NM_001014594.3	NP_001014594.1	2 - 3	1072	67
	NM_001014595.3	NP_001014595.1	3 - 4	1007	67
	NM_001014596.3	NP_001014596.1	3 - 4	677	67
	NM_001014597.2	NP_001014597.1	2 - 3	505	67
	NM_001014598.2	NP_001014598.1	2 - 3	752	67
	NM_001202215.1	NP_001189144.1	3 - 4	628	67
	NM_001202216.1	NP_001189145.1	2 - 3	679	67
	NM_001202217.1	NP_001189146.1	2 - 3	752	67
	NM_001202218.2	NP_001189147.1	2 - 3	1072	67
	NM_001202219.2	NP_001189148.1	3 - 4	1014	67
	NM_001202220.2	NP_001189149.1	3 - 4	677	67
	NM_001202221.1	NP_001189150.1	2 - 3	861	67
	NM_079465.4	NP_524189.2	2 - 3	679	67
	NM_141006.4	NP_649263.2	2 - 3	861	67
	NM_168875.4	NP_730573.2	2 - 3	1072	67
	NM_168876.6	NP_730574.2	3 - 4	1014	67
	NM_168877.2	NP_730576.1	3 - 4	628	67
GenBank mRNA	AY058709.1	-	2 - 3	1038	67
	AY122131.1	-	2 - 3	679	67
	BT133037.1	-	3 - 4	628	67