Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pka-R1
Entrez Gene ID: 40305
Gene Name: Protein kinase, cAMP-dependent, regulatory subunit type 1
Gene Aliases: 18304, CG12452, CG18677, CG3263, CG42341, CdkR, DRI, Dmel\CG42341, Dmel_CG42341, PKA, PKA R1, PKA-R1, PKA-RI, PKAR, PKARI, PKAi, PKAr, PKa-R1, Pka-RI, PkaR, RI, dPKA-RI, dr[[1]], pKA, pkA, pka-R1
Chromosome Location: Chr.3L: 20816118 - 20836048 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0259243

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001014593.3	NP_001014593.1	4 - 5	1586	59
	NM_001014594.3	NP_001014594.1	4 - 5	1586	59
	NM_001014595.3	NP_001014595.1	5 - 6	1521	59
	NM_001014596.3	NP_001014596.1	5 - 6	1191	59
	NM_001014597.2	NP_001014597.1	4 - 5	1019	59
	NM_001014598.2	NP_001014598.1	4 - 5	1266	59
	NM_001202215.1	NP_001189144.1	5 - 6	1145	59
	NM_001202216.1	NP_001189145.1	4 - 5	1196	59
	NM_001202217.1	NP_001189146.1	4 - 5	1269	59
	NM_001202218.2	NP_001189147.1	4 - 5	1589	59
	NM_001202219.2	NP_001189148.1	5 - 6	1531	59
	NM_001202220.2	NP_001189149.1	5 - 6	1194	59
	NM_001202221.1	NP_001189150.1	4 - 5	1378	59
	NM_079465.4	NP_524189.2	4 - 5	1193	59
	NM_141006.4	NP_649263.2	4 - 5	1375	59
	NM_168875.4	NP_730573.2	4 - 5	1586	59
	NM_168876.6	NP_730574.2	5 - 6	1528	59
	NM_168877.2	NP_730576.1	5 - 6	1142	59
GenBank mRNA	AY058709.1	-	4 - 5	1552	59
	AY122131.1	-	4 - 5	1193	59
	BT133037.1	-	5 - 6	1145	59