Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: fwd
Entrez Gene ID: 45374
Gene Name: four wheel drive
Gene Aliases: CG7004, Dmel\CG7004, Dmel_CG7004, Fwd, PI4K, PI4K3B, PI4KIIIbeta, anon-WO0118547.259
Chromosome Location: Chr.3L: 305393 - 319884 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0004373

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001274274.1	NP_001261203.1	12 - 13	5790	101
	NM_001370043.1	NP_001356971.1	11 - 12	6023	101
	NM_080083.4	NP_524822.1	11 - 12	6023	101
	NM_167826.2	NP_728518.1	11 - 12	5267	101
	NM_167827.2	NP_728519.1	10 - 11	4096	101
GenBank mRNA	AF242375.2	-	10 - 11	4096	101
GenBank mRNA	AY052049.1	-	11 - 12	6004	101