Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: CkIIalpha
Entrez Gene ID: 48448
Gene Name: casein kinase IIalpha
Gene Aliases: 0958/08, CG17520, CJIIalpha, CK II, CK-2, CK-II, CK-II alpha, CK2, CK2 alpha, CK2-alpha, CK2a, CK2alpha, CKII, CKII alpha, CKII-alpha, CKIIa, CKIIalfa, CKIIalpha, Cask-II-a, Ck2alpha, CkII, CkIIa, Dm-CK2-alpha, DmCK2alpha, DmCKIIalpha, Dmel\CG17520, Dmel_CG17520, Tik, alphaCK2, anon-WO02059370.53, ck2alpha, ckIIalpha, dCK2alpha, dCKII, dCKII alpha, l(3)S095808
Chromosome Location: Chr.3L: 23097072 - 23103460 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0264492

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001043159.3	NP_001036624.1	3 - 3	1314	136
	NM_001300222.1	NP_001287151.1	2 - 2	1214	136
	NM_001300223.1	NP_001287152.1	2 - 2	1162	136
	NM_001300224.1	NP_001287153.1	4 - 4	1786	136
	NM_080179.5	NP_524918.1	3 - 3	1294	136
	NM_168985.2	NP_730774.1	3 - 3	1269	136
	NM_168986.2	NP_730775.1	2 - 2	1156	136
GenBank mRNA	AY069553.1	-	3 - 3	1210	136
GenBank mRNA	M16534.1	-	3 - 3	1200	136