The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_131273.1 | NP_571348.1 | 1 - 1 | 256 | 87 |
| GenBank mRNA | BC095601.1 | - | 1 - 1 | 284 | 113 |
| BC162283.1 | - | 1 - 1 | 309 | 87 | |
| BC162290.1 | - | 1 - 1 | 309 | 87 | |
| U50563.1 | - | 1 - 1 | 256 | 87 | |