The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001292048.1 | NP_001278977.1 | 3 - 3 | 772 | 83 |
| NM_004162.4 | NP_004153.2 | 3 - 3 | 814 | 83 | |
| GenBank mRNA | AF464088.1 | - | 3 - 3 | 655 | 83 |
| AF498936.1 | - | 2 - 2 | 279 | 83 | |
| AK295992.1 | - | 3 - 3 | 447 | 83 | |
| AK309287.1 | - | 4 - 4 | 665 | 83 | |
| AK312618.1 | - | 3 - 3 | 528 | 83 | |
| BC001267.1 | - | 3 - 3 | 444 | 83 | |
| BC018288.1 | - | 3 - 3 | 497 | 83 | |
| CR536492.1 | - | 2 - 2 | 279 | 83 | |
| M28215.1 | - | 2 - 2 | 335 | 83 | |
| X13903.1 | - | 2 - 2 | 279 | 83 | |