The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001311311.1 | NP_001298240.1 | 4 - 4 | 248 | |
| NM_024006.5 | NP_076869.1 | 3 - 3 | 561 | 164 | |
| GenBank mRNA | AF176924.1 | - | 4 - 4 | 246 | |
| AK312005.1 | - | 3 - 3 | 474 | 164 | |
| AY423044.1 | - | 3 - 3 | 291 | 164 | |
| AY466113.1 | - | 3 - 3 | 503 | 164 | |
| AY521634.1 | - | 3 - 3 | 291 | 164 | |
| BC000828.1 | - | 3 - 3 | 291 | 164 | |
| BC002911.2 | - | 3 - 3 | 329 | 164 | |
| BI822140.1 | - | 3 - 3 | 517 | 164 | |
| BM663008.1 | - | 1 - 1 | 163 | ||
| CN484751.1 | - | 4 - 4 | 248 | ||
| KU178762.1 | - | 3 - 3 | 291 | 164 | |