The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001135.3 | NP_001126.3 | 1 - 1 | 355 | 76 |
| NM_013227.3 | NP_037359.3 | 1 - 1 | 355 | 76 | |
| XM_006720419.1 | XP_006720482.1 | 1 - 1 | 356 | 76 | |
| XM_011521313.1 | XP_011519615.1 | 1 - 1 | 356 | 76 | |
| XM_011521314.1 | XP_011519616.1 | 1 - 1 | 356 | 76 | |
| XM_017021985.1 | XP_016877474.1 | 1 - 1 | 356 | 76 | |
| XM_017021986.1 | XP_016877475.1 | 1 - 1 | 356 | 76 | |
| XM_017021987.1 | XP_016877476.1 | 1 - 1 | 356 | 76 | |
| GenBank mRNA | AK290723.1 | - | 1 - 1 | 355 | 76 |
| BC036445.1 | - | 1 - 1 | 326 | 76 | |
| BC150624.1 | - | - | 39 | 76 | |
| DA596050.1 | - | 1 - 1 | 355 | 76 | |
| M55172.1 | - | 1 - 1 | 41 | 76 | |