Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001256648.1	NP_001243577.1	7 - 7	1316	148
	NM_001256649.1	NP_001243578.1	7 - 7	1181	148
	NM_001256650.1	NP_001243579.1	6 - 6	1053	148
	NM_001256651.1	NP_001243580.1	3 - 3	667	148
	NM_001256653.1	NP_001243582.1	4 - 4	794	148
	NM_001256654.1	NP_001243583.1	3 - 3	676	148
	NM_003423.3	NP_003414.2	4 - 4	803	148
	XM_011528257.2	XP_011526559.1	4 - 4	725	148
	XM_011528259.2	XP_011526561.1	4 - 4	1282	148
	XM_017027207.1	XP_016882696.1	7 - 7	6794	148
	XM_017027208.1	XP_016882697.1	7 - 7	1474	148
	XM_017027209.1	XP_016882698.1	7 - 7	6527	148
	XM_017027210.1	XP_016882699.1	6 - 6	6318	148
	XM_017027211.1	XP_016882700.1	7 - 7	6461	148
	XM_017027212.1	XP_016882701.1	6 - 6	1321	148
	XM_017027213.1	XP_016882702.1	7 - 7	6569	148
	XM_017027214.1	XP_016882703.1	5 - 5	1244	148
	XM_017027215.1	XP_016882704.1	6 - 6	1083	148
	XM_017027216.1	XP_016882705.1	5 - 5	869	148
GenBank mRNA	AF112859.1	-	1 - 1	220	148
	AK291353.1	-	7 - 7	1131	148
	BC006528.2	-	4 - 4	770	148
	BT009756.1	-	3 - 3	615	148
	CR933640.1	-	7 - 7	1276	148
	X59244.1	-	6 - 6	952	148