The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001256799.2 | NP_001243728.1 | 7 - 7 | 807 | 139 |
| NM_001289745.1 | NP_001276674.1 | 8 - 8 | 865 | 139 | |
| NM_001289746.1 | NP_001276675.1 | 7 - 7 | 759 | 139 | |
| NM_002046.5 | NP_002037.2 | 8 - 8 | 773 | 139 | |
| GenBank mRNA | AB062273.1 | - | 8 - 8 | 637 | 139 |
| AF261085.1 | - | 8 - 8 | 688 | 139 | |
| AK026525.1 | - | 8 - 8 | 710 | 139 | |
| AK308198.1 | - | 8 - 8 | 661 | 139 | |
| AY007133.1 | - | 8 - 8 | 637 | 139 | |
| AY633612.1 | - | 7 - 7 | 609 | 139 | |
| BC001601.1 | - | 8 - 8 | 639 | 139 | |
| BC004109.2 | - | 8 - 8 | 637 | 139 | |
| BC009081.1 | - | 8 - 8 | 646 | 139 | |
| BC013310.2 | - | 8 - 8 | 645 | 139 | |
| BC020308.1 | - | 7 - 7 | 623 | 139 | |
| BC023632.2 | - | 7 - 7 | 609 | 139 | |
| BC025925.1 | - | 8 - 8 | 642 | 139 | |
| BC026907.1 | - | 7 - 7 | 623 | 139 | |
| BC029340.1 | - | 8 - 8 | 631 | 139 | |
| BC029618.1 | - | 8 - 8 | 639 | 139 | |
| BC083511.1 | - | 8 - 8 | 637 | 139 | |
| BT006893.1 | - | 7 - 7 | 585 | 139 | |
| CR407671.1 | - | 7 - 7 | 585 | 139 | |
| DQ403057.1 | - | 6 - 6 | 510 | 139 | |
| EF036498.1 | - | 4 - 4 | 323 | 139 | |
| EU668321.1 | - | 8 - 8 | 687 | 139 | |
| J02642.1 | - | 7 - 7 | 586 | 139 | |
| M17851.1 | - | 7 - 7 | 618 | 139 | |
| M33197.1 | - | 8 - 8 | 645 | 139 | |
| X01677.1 | - | 7 - 7 | 645 | 139 | |
| X53778.1 | - | 8 - 8 | 138 | ||