Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: DMD
Entrez Gene ID: 1756
Gene Name: dystrophin
Gene Aliases: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
Chromosome Location: Chr.X: 31119219 - 33339609 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000109.3	NP_000100.2	74 - 74	10777	95
	NM_004006.2	NP_003997.1	74 - 74	10701	95
	NM_004009.3	NP_004000.1	74 - 74	10708	95
	NM_004010.3	NP_004001.1	74 - 74	10791	95
	NM_004011.3	NP_004002.2	46 - 46	6479	95
	NM_004012.3	NP_004003.1	46 - 46	6622	95
	NM_004013.2	NP_004004.1	31 - 31	4118	95
	NM_004014.2	NP_004005.1	20 - 20	2331	95
	NM_004015.2	NP_004006.1	13 - 13	1331	95
	NM_004016.2	NP_004007.1	13 - 13	1331	95
	NM_004017.2	NP_004008.1	12 - 12	1292	95
	NM_004018.2	NP_004009.1	12 - 12	1292	95
	NM_004021.2	NP_004012.1	31 - 31	4118	95
	NM_004022.2	NP_004013.1	30 - 30	4079	95
	XM_006724468.2	XP_006724531.1	74 - 74	10654	95
	XM_006724469.3	XP_006724532.1	74 - 74	10628	95
	XM_006724470.3	XP_006724533.1	73 - 73	10615	95
	XM_006724473.2	XP_006724536.1	73 - 73	10516	95
	XM_011545467.1	XP_011543769.1	73 - 73	10531	95
	XM_017029328.1	XP_016884817.1	73 - 73	10615	95
	XM_017029331.1	XP_016884820.1	34 - 34	5230	95
GenBank mRNA	AB208836.1	-	27 - 27	3526	95
	BC028720.1	-	13 - 13	1382	95
	BC070078.1	-	12 - 12	1293	95
	BC094758.1	-	13 - 13	1345	95
	BC127103.1	-	28 - 28	3642	95
	BC150141.1	-	28 - 28	3642	95
	KU178030.1	-	13 - 13	1253	95
	KU178031.1	-	13 - 13	1253	95
	M18533.1	-	74 - 74	10665	95
	M92650.1	-	12 - 12	1266	95
	X14298.1	-	74 - 74	10555	95