Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: DMD
Entrez Gene ID: 1756
Gene Name: dystrophin
Gene Aliases: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
Chromosome Location: Chr.X: 31119219 - 33339609 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000109.3	NP_000100.2	59 - 59	9094	141
	NM_004006.2	NP_003997.1	59 - 59	9018	141
	NM_004009.3	NP_004000.1	59 - 59	9025	141
	NM_004010.3	NP_004001.1	59 - 59	9108	141
	NM_004011.3	NP_004002.2	31 - 31	4796	141
	NM_004012.3	NP_004003.1	31 - 31	4939	141
	NM_004013.2	NP_004004.1	16 - 16	2435	141
	NM_004014.2	NP_004005.1	5 - 5	648	141
	NM_004020.3	NP_004011.2	16 - 16	2435	141
	NM_004021.2	NP_004012.1	16 - 16	2435	141
	NM_004022.2	NP_004013.1	16 - 16	2435	141
	NM_004023.2	NP_004014.1	16 - 16	2435	141
	XM_006724468.2	XP_006724531.1	59 - 59	8971	141
	XM_006724469.3	XP_006724532.1	59 - 59	8945	141
	XM_006724470.3	XP_006724533.1	59 - 59	8971	141
	XM_006724473.2	XP_006724536.1	58 - 58	8833	141
	XM_006724474.3	XP_006724537.1	59 - 59	8971	141
	XM_006724475.2	XP_006724538.1	59 - 59	8971	141
	XM_011545467.1	XP_011543769.1	58 - 58	8848	141
	XM_011545468.2	XP_011543770.1	59 - 59	8971	141
	XM_017029328.1	XP_016884817.1	59 - 59	8971	141
	XM_017029331.1	XP_016884820.1	19 - 19	3547	141
GenBank mRNA	AB208836.1	-	13 - 13	1882	141
	BC127103.1	-	13 - 13	1959	141
	BC150141.1	-	13 - 13	1959	141
	M18533.1	-	59 - 59	8982	141
	X14298.1	-	59 - 59	8872	141