The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001287813.2 | NP_001274742.1 | 20 - 20 | 1970 | 74 |
| NM_001287814.2 | NP_001274743.1 | 17 - 17 | 2011 | 74 | |
| NM_001321773.1 | NP_001308702.1 | 18 - 18 | 2122 | 74 | |
| NM_018967.4 | NP_061840.1 | 19 - 19 | 2197 | 74 | |
| NR_135794.1 | 19 - 19 | 2177 | 74 | ||
| NR_135795.1 | 19 - 19 | 2192 | 74 | ||
| NR_135796.1 | 19 - 19 | 2223 | 74 | ||
| NR_135797.1 | 21 - 21 | 2367 | 74 | ||
| XM_011517548.2 | XP_011515850.1 | 18 - 18 | 2307 | 74 | |
| XM_017013579.1 | XP_016869068.1 | 21 - 21 | 5126 | 74 | |
| XM_017013580.1 | XP_016869069.1 | 19 - 19 | 1720 | 74 | |
| GenBank mRNA | AJ003030.1 | - | 17 - 17 | 1647 | 74 |
| AL161971.1 | - | 19 - 19 | 2092 | 74 | |
| BC075072.2 | - | 17 - 17 | 1562 | 74 | |
| BC104829.1 | - | 18 - 18 | 1644 | 74 | |
| BC143695.1 | - | 18 - 18 | 1670 | 74 | |