The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001013435.2 | NP_001013453.1 | 1 - 1 | 56 | 133 |
| GenBank mRNA | BP429046.1 | - | 1 - 1 | 60 | 133 |
| BQ437151.1 | - | 1 - 1 | 44 | 133 | |