The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000519.3 | NP_000510.1 | 3 - 3 | 528 | 170 |
| GenBank mRNA | AY034468.1 | - | 3 - 3 | 528 | 170 |
| BC069307.1 | - | 3 - 3 | 383 | 170 | |
| BC070282.1 | - | 3 - 3 | 384 | 170 | |
| BU664913.1 | - | 3 - 3 | 347 | 170 | |