The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000600.4 | NP_000591.1 | 4 - 4 | 562 | 146 |
| NM_001318095.1 | NP_001305024.1 | 3 - 3 | 371 | 146 | |
| XM_011515390.2 | XP_011513692.1 | 5 - 5 | 895 | 146 | |
| GenBank mRNA | A09363.1 | - | 4 - 4 | 146 | |
| BC015511.1 | - | 4 - 4 | 490 | 146 | |
| BT019748.1 | - | 4 - 4 | 441 | 146 | |
| BT019749.1 | - | 4 - 4 | 441 | 146 | |
| CD013918.1 | - | 3 - 3 | 299 | 146 | |
| CR450296.1 | - | 4 - 4 | 441 | 146 | |
| M14584.1 | - | 4 - 4 | 146 | ||
| M18403.1 | - | 4 - 4 | 503 | 146 | |
| M29150.1 | - | 4 - 4 | 476 | 146 | |
| M54894.1 | - | 4 - 4 | 491 | 146 | |
| S56892.1 | - | 4 - 4 | 456 | 146 | |
| X04403.1 | - | 3 - 3 | 348 | 146 | |
| X04430.1 | - | 4 - 4 | 146 | ||
| X04602.1 | - | 4 - 4 | 474 | 146 | |