The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001035512.1 | NP_001030589.1 | 2 - 2 | 79 | 75 |
NM_001278172.1 | NP_001265101.1 | 2 - 2 | 79 | 75 | |
GenBank mRNA | AB211234.1 | - | 2 - 2 | 73 | 75 |
AB212048.1 | - | 2 - 2 | 67 | 75 |