The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001244937.2 | NP_001231866.1 | 16 - 16 | 3477 | 98 |
| NM_001318913.1 | NP_001305842.1 | 15 - 15 | 3306 | 98 | |
| NM_002447.3 | NP_002438.2 | 17 - 17 | 3624 | 98 | |
| NR_134919.1 | 17 - 17 | 3560 | 98 | ||
| XM_005265170.4 | XP_005265227.2 | 17 - 17 | 3622 | 98 | |
| XM_011533739.2 | XP_011532041.1 | 17 - 17 | 3502 | 98 | |
| XM_011533740.2 | XP_011532042.1 | 16 - 16 | 3475 | 98 | |
| XM_011533741.2 | XP_011532043.1 | 16 - 16 | 3415 | 98 | |
| XM_011533742.2 | XP_011532044.1 | 17 - 17 | 3622 | 98 | |
| XM_011533744.2 | XP_011532046.1 | 15 - 15 | 3304 | 98 | |
| GenBank mRNA | AK303234.1 | - | 7 - 7 | 964 | 98 |
| HQ908652.1 | - | 17 - 17 | 3532 | 98 | |
| JN689381.1 | - | 3 - 3 | 158 | 98 | |
| JN983806.1 | - | 15 - 15 | 3306 | 98 | |
| X70040.1 | - | 17 - 17 | 3624 | 98 | |