Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
Gad1
Entrez Gene ID
14415
Gene Name
glutamate decarboxylase 1
Gene Aliases
EP10, GAD25, GAD44, GAD67, Gad-1
Chromosome Location
Chr.2: 70561763 - 70602014 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_008077.4 NP_032103.2 16 - 16 1799 66
GenBank mRNA AF326547.1 - 16 - 16 1749 66
AF326548.1 - 16 - 16 1749 66
AF483492.1 - 15 - 15 1566 66
AF483493.1 - 15 - 15 1566 66
AK136801.1 - 16 - 16 1784 66
AK148497.1 - 16 - 16 1799 66
AK158827.1 - 15 - 15 1565 66
AK160410.1 - 15 - 15 1708 66
AK160868.1 - 16 - 16 1797 66
AK163893.1 - 2 - 2 144 66
BC027059.1 - 16 - 16 1750 66
KC134210.1 - 15 - 15 1566 66
Y12257.1 - 15 - 15 1566 66
Z49976.1 - 16 - 16 1686 66

Gene Symbol
Gad1-ps
Entrez Gene ID
382407
Gene Name
glutamate decarboxylase 1, pseudogene
Gene Aliases
Gad-1ps
Chromosome Location
Chr.10: 99443838 - 99447029 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
GenBank mRNA AK137453.1 - 1 - 1 1875 66
AK137640.1 - 1 - 1 1899 66

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