The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001083126.1 | NP_001076595.1 | 8 - 8 | 1121 | 150 |
| NM_008500.2 | NP_032526.2 | 9 - 9 | 1311 | 150 | |
| GenBank mRNA | AB031040.1 | - | 8 - 8 | 1121 | 150 |
| AJ000337.1 | - | 9 - 9 | 1148 | 148 | |
| BC065077.1 | - | 8 - 8 | 1055 | 150 | |