The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001164574.1 | NP_001158046.1 | 2 - 2 | 427 | 94 |
| NM_026962.3 | NP_081238.2 | 3 - 3 | 459 | 94 | |
| GenBank mRNA | AK008627.1 | - | 2 - 2 | 317 | 94 |
| AK034334.1 | - | 3 - 3 | 367 | 94 | |
| AK078744.1 | - | 2 - 2 | 339 | 94 | |
| AK083497.1 | - | 3 - 3 | 441 | 94 | |
| AK168345.1 | - | 2 - 2 | 273 | 94 | |
| BB264869.2 | - | 2 - 2 | 428 | 94 | |
| BC024938.1 | - | 2 - 2 | 323 | 94 | |
| BC027392.1 | - | 3 - 3 | 363 | 94 | |
| BE650264.1 | - | 3 - 3 | 460 | 94 | |