The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_029344.3 | NP_083620.1 | 3 - 3 | 147 | 152 |
GenBank mRNA | AK009134.1 | - | 3 - 3 | 147 | 152 |
AK046238.1 | - | 3 - 3 | 156 | 152 | |
BC027642.1 | - | 3 - 3 | 135 | 152 |