Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Nfkb1
Entrez Gene ID: 18033
Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Gene Aliases: NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105
Chromosome Location: Chr.3: 135584655 - 135691547 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_008689.2	NP_032715.2	19 - 20	2607	91
GenBank mRNA	AB119195.1	-	17 - 18	2105	91
	AB221614.1	-	17 - 18	2112	91
	AK089660.1	-	7 - 8	981	91
	AK146389.1	-	5 - 6	826	91
	AK157915.1	-	4 - 5	607	91
	AK159423.1	-	7 - 8	1038	91
	AY521462.1	-	17 - 18	2105	91
	AY521463.1	-	17 - 18	2105	91
	BC138535.1	-	18 - 19	2184	91
	BC138536.1	-	18 - 19	2184	91
	BC145317.1	-	19 - 20	2201	91
	L28117.1	-	7 - 8		90
	M57999.1	-	19 - 20	2396	91
	S66656.1	-	7 - 8	1151	91
	S89033.1	-	7 - 8	1199	91