Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Six3
Entrez Gene ID: 20473
Gene Name: sine oculis-related homeobox 3
Gene Aliases: E130112M24Rik, Six3a, Six3alpha, Six3b, Six3beta
Chromosome Location: Chr.17: 85620834 - 85626191 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_011381.4	NP_035511.2	2 - 2		105
GenBank mRNA	AK021385.1	-	1 - 1	149	106

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	CK622937.1	-	1			Chr. 17 - 85624107 - 85624603 [+] on Build GRCm38	105
GenBankmRNA	BY731327.1	-		149		Chr. - - [-] on Build	106
GenBankmRNA	CK627912.1	-	1			Chr. 17 - 85624330 - 85624920 [+] on Build GRCm38	105

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	SIX homeobox 3, opposite strand 1	Six3os1	D17Mgi26, E130112H22Rik, Rncr1, Six3os	-5.9kb	Anti-sense	Chr. - 85601932 - 85618396 on Build GRCm38
Downstream	sine oculis-related homeobox 2	Six2		37.5kb	Anti-sense	Chr. - 85662735 - 85688254 on Build GRCm38

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