The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_011016.2 | NP_035146.1 | 3 - 3 | 357 | 97 |
| GenBank mRNA | BC057985.1 | - | 3 - 3 | 332 | 97 |
| CB949119.1 | - | 3 - 3 | 360 | 97 | |
| M12566.1 | - | 2 - 2 | 112 | 97 | |
| M27009.1 | - | 3 - 3 | 322 | 97 | |