The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_031565.2 | NP_113753.2 | 3 - 3 | 604 | 72 |
| GenBank mRNA | EX493673.1 | - | 3 - 4 | 396 | 72 |
| X81395.1 | - | 3 - 3 | 469 | 72 | |