The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001127558.1 | NP_001121030.1 | 5 - 5 | 790 | 141 |
| XM_008767695.1 | XP_008765917.1 | 6 - 6 | 854 | 141 | |
| XM_008767697.1 | XP_008765919.1 | 6 - 6 | 862 | 141 | |
| XM_008767698.1 | XP_008765920.1 | 6 - 6 | 815 | 141 | |
| XM_008767699.1 | XP_008765921.1 | 6 - 6 | 818 | 141 | |
| GenBank mRNA | BC161925.1 | - | 5 - 5 | 790 | 141 |