The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_053614.1 | NP_446066.1 | 1 - 1 | 250 | 110 |
GenBank mRNA | U15149.1 | - | 1 - 1 | 250 | 110 |