The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001271731.1 | NP_001258660.1 | 5 - 5 | 522 | 68 |
GenBank mRNA | CB691171.1 | - | 3 - 3 | 120 | 68 |