The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_017008.4 | NP_058704.1 | 3 - 3 | 292 | 87 |
| GenBank mRNA | AB017801.1 | - | 3 - 3 | 258 | 87 |
| BC059110.1 | - | 3 - 3 | 293 | 87 | |
| BC087743.1 | - | 3 - 3 | 261 | 87 | |
| DQ403053.1 | - | 1 - 1 | 148 | 87 | |
| M17701.1 | - | 2 - 2 | 247 | 87 | |
| X02231.1 | - | 3 - 3 | 288 | 87 | |