The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001167631.1 | NP_001161103.1 | 8 - 8 | 828 | 105 |
| XM_005661256.3 | XP_005661313.1 | - | 1125 | 105 | |
| GenBank mRNA | AB529856.1 | - | 8 - 8 | 828 | 105 |
| AK233295.1 | - | 9 - 9 | 911 | 105 | |
| AK345947.1 | - | 9 - 9 | 911 | 105 | |
| AK396912.1 | - | 9 - 9 | 1002 | 105 | |
| EU275148.1 | - | 6 - 6 | 551 | 105 | |