The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001097457.1 | NP_001090926.1 | 6 - 6 | 2252 | 79 |
| XM_021074490.1 | XP_020930149.1 | - | 2637 | 79 | |
| XM_021074491.1 | XP_020930150.1 | - | 2306 | 79 | |
| XM_021074492.1 | XP_020930151.1 | - | 2562 | 79 | |
| XM_021074493.1 | XP_020930152.1 | - | 2659 | 104 | |
| GenBank mRNA | EF065517.1 | - | 6 - 6 | 2252 | 79 |