The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001097472.1 | NP_001090941.1 | 3 - 3 | 254 | 111 |
| GenBank mRNA | AK400022.1 | - | 3 - 3 | 257 | 111 |
| DQ629150.1 | - | 3 - 3 | 254 | 111 | |